Human COQ10A (Coenzyme Q-binding protein COQ10 homolog A, mitochondrial) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9871-48, ELK9871-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Coenzyme Q-binding protein COQ10 homolog A, mitochondrial, COQ10A
Species: Human
Assay Type: Sandwich
Sensitivity: 0.131 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Enzyme and Kinase,
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COQ10A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COQ10A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COQ10A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COQ10A in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: Q96MF6
Research Use Only