Human FSTL3 (Follistatin Like Protein 3) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9833-48, ELK9833-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: FLRG, FSRP, Follistatin-Related Protein,Secreted Glycoprotein, Follistatin-related gene protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Reproductive science,
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FSTL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FSTL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FSTL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FSTL3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: O95633
Research Use Only