Mouse Tb4 (Thymosin Beta 4) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9849-48, ELK9849-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TMSB4X, FX, PTMB4, TB4X, TMSB4, Thymosin,Beta 4,X-Linked, TB-500, Seraspenide, Hematopoietic system regulatory peptide
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 0.87 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway, Endocrinology, Bone metabolism,
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Tb4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: P20065
Research Use Only