DNA topoisomerase I (TOP1) Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A12409-20, A12409-100
Citations, Manuals and SDS Available upon request.
Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Synonyms: TOPI; DNA topoisomerase I (TOP1)
GeneID: 7150
Swiss Prot: P11387
Tested Applications: ELISA, WB, IHC-P, IF/ICC, IP
Reactivity: Human, Mouse
Calculated Molecular Weight: 91kDa
Observed Molecular Weight: 100kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (TOP1) (P11387).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Research Areas: Epigenetics Nuclear Signaling, RNA Binding, Cancer
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: Mouse thymus, HeLa, MCF7, NIH/3T3, K-562
Cellular Location: Nucleus, Nucleolus, Nucleoplasm,
Keywords: TOP1, TOPI, topoisomerase (DNA) I, DNA topoisomerase 1|type I DNA topoisomerase, P11387, DNA topoisomerase I (TOP1)
Symbol: TOP1
Category: Monoclonal Antibodies
Research Use Only