PDHA1 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A13687-20, A13687-100
Citations, Manuals and SDS Available upon request.
Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Synonyms: PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; PDHA1
GeneID: 5160
Swiss Prot: P08559
Tested Applications: ELISA, WB, IF/ICC
Reactivity: Human, Mouse, Rat
Calculated Molecular Weight: 43kDa
Observed Molecular Weight: 43kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PDHA1 (P08559).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:2000 | IF/ICC: 1:50 - 1:200
Research Areas: Cancer, Signal Transduction, Endocrine Metabolism, Mitochondrial metabolism, Mitochondrial markers, Carbohydrate metabolism, Warburg Effect
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: HeLa, 293T, HepG2, Mouse liver, Rat liver
Cellular Location: Mitochondrion Matrix
Keywords: PDHA1, PDHA, PDHAD, PDHCE1A, PHE1A, pyruvate dehydrogenase (lipoamide) alpha 1, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial|PDHE1-A type I|pyruvate dehydrogenase E1 subunit|pyruvate dehydrogenase complex, E1-alpha polypeptide 1, P08559
Symbol: PDHA1
Category: Monoclonal Antibodies
Research Use Only