Sonic Hedgehog (Shh) Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A12695-20, A12695-100
Citations, Manuals and SDS Available upon request.
Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
Synonyms: TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5; Sonic Hedgehog (Shh)
GeneID: 6469
Swiss Prot: Q15465
Tested Applications: ELISA, WB, IF/ICC
Reactivity: Human, Mouse, Rat
Calculated Molecular Weight: 50kDa
Observed Molecular Weight: 50kDa/27kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Sonic Hedgehog (Shh) (Q15465).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:2000 | IF/ICC: 1:50 - 1:200
Research Areas: Epigenetics Nuclear Signaling, Cancer, Tumor suppressors, Cell Biology Developmental Biology, Endocrine Metabolism, Stem Cells, Embryonic Stem Cells, Neural Stem Cells, Cardiovascular, Angiogenesis
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: HepG2, Mouse lung, Mouse liver, Rat liver, Human plasma
Cellular Location: Cell Membrane, Lipid-anchor, Secreted, Extra Cellular Space,
Keywords: SHH, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, sonic hedgehog, sonic hedgehog protein|sonic hedgehog homolog, Q15465, Sonic Hedgehog (Shh)
Symbol: SHH
Category: Monoclonal Antibodies
Research Use Only