The easy and efficient reagent for transfection of mammalian cells with DNA
Catalogue Numbers:
- T090-1.0 (1 x 1ml)
- T090-2.0 (2 x 1ml)
- T090-5.0 (5 x 1ml)
- EASY-Buffer - EA010-2.0 (1 x 2 ml)
Sizes:
- 1 x 1ml (T090-1.0)
- 2 x 1ml (T090-2.0)
- 5 x 1ml (T090-5.0)
- EASY-Buffer - 1 x 2 ml (EA010-2.0)
HIGHLIGHTS:
- Suitable for DNA transfection
- Easy: Optimization involves only two fixed DNA:lipid ratios
- Fast: Results within 2-3 days → two interdependent assays within a week
- Efficient for a majority of cell types
Common transfection reagents require considerable efforts to optimize the DNA:lipid ratio and the amount of DNA used and, as a rule, involve considerable expenditure of time – 4 days – from seeding the cells to reporter gene assay. The idea behind the development of METAFECTENE® EASY⁺ was to establish an efficient transfection reagent combined with a fast and easy protocol.
METAFECTENE® EASY⁺ works with two predefined DNA/lipid ratios and a predefined amount of lipoplexes combined with a "Fast Forward" protocol. This enables high transfection efficiencies to be reached for a variety of robust mammalian cell lines and primary cells while minimizing optimization effort.
MAIN ADVANTAGES OF THE "FAST FORWARD" PROTOCOL:
Using the "Fast Forward" protocol, cells are seeded and subsequently transfected without being allowed to grow on the vessel surface by incubation overnight. This results in a time saving of 24 hours, allowing two successive result-dependent transfections to be performed within a single working week.