AANAT Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS60541-50, BS60541-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q16613
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: AANAT (Aralkylamine N-acetyltransferase), also called Serotonin N-acetyltransferase (SNAT) or Serotonin acetylase, is a member of the acetyltransferase superfamily. It is almost exclusively expressed in the pineal gland and the retina. AANAT activity is high at night and low during the day due to the 10 to 100-fold increase in the amount of active AANAT at night. Retinal exposure to light causes cAMP levels to decrease in photoreceptor cells and, as a result, AANAT is targeted for degradation by proteasomal proteolysis. AANAT plays an important role as the rate limiting enzyme in melatonin synthesis. It is responsible for catalyzing the N-acetylation of serotonin to N-acetylserotonin, which is then converted to melatonin by hydroxyindole-O-methyltransferase. Melatonin is an important hormone that is involved in many physiological processes including immune function, seasonal reproduction, retinal physiology and circadian entrainment.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AANAT polyclonal antibody detects endogenous levels of AANAT protein.
Bioworld Molecular Weight: ~ 23 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AANAT polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of AANAT pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic strong positive staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of AANAT pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic strong positive staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Serotonin N-acetyltransferase; Serotonin acetylase; Aralkylamine N-acetyltransferase; AA-NAT; AANAT; SNAT
Immunogen: A synthetic peptide corresponding to residues in Human AANAT
Conjugate: Unconjugated
Modification: Unmodified