CHRFAM7A Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50µl, 100µl
Catalogue Numbers: BS74925-50, BS74925-100
Swiss-Prot: Q494W8
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 50kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using CHRFAM7A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Alternative Name: CHRFAM7A; CHRNA7; CHRNA7-DR1; D-10
Immunogen: Recombinant fusion protein of human CHRFAM7A (NP_647536.1).
Conjugate: Unconjugated
Modification: Unmodified