{"title":"Cell Metabolism Assays","description":null,"products":[{"product_id":"revertose-colorimetric-assay-kit-dns-method-e-bc-k757-m-48","title":"Revertose Colorimetric Assay Kit (DNS Method) - E-BC-K757-M-48","description":"\u003cp\u003eRevertose Colorimetric Assay Kit (DNS Method)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K757-M-48, E-BC-K757-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at 2-8°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas:  Plant Physiology Series\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.036-1.0 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum;Plasma;Animal and plant tissue\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 50 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 0.036 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Revertose are sugars with reducing properties. In sugars, both monosaccharides containing free aldehyde or ketone groups and disaccharides containing free aldehyde groups are reductive. Revertose mainly include glucose, fructose, galactose, lactose, maltose, etc. Revertose are reduced to amino compounds after co-heating with 3, 5-dinitrosalicylic acid under alkaline conditions, and are reddish brown in alkaline solution. The amount of revertose is proportional to the depth of color of reddish-brown substances, so as to determine the content of revertose in samples.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 2.1-5.3%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 1.0-2.9%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896277655731,"sku":"E-BC-K757-M-48","price":280.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896277688499,"sku":"E-BC-K757-M-96","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"enhanced-cell-malondialdehyde-mda-colorimetric-assay-kit-e-bc-k814-m-48","title":"Enhanced Cell Malondialdehyde (MDA) Colorimetric Assay Kit - E-BC-K814-M-48","description":"\u003cp\u003eEnhanced Cell Malondialdehyde (MDA) Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K814-M-48, E-BC-K814-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: MDA\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at 2-8°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas:  Oxidative Stress\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.19-100.00 nmol\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Cell\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 1 h 20 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 0.19 nmol\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Malondialdehyde (MDA) in the degradation products of peroxidized lipids can react with thiobarbituric acid (TBA) under high temperature and acidic conditions to produce the red-brown product 3,5,5 '-trimethyloxazole-2, 4-dione (trimethine), and this substance has the maximum absorption peak at 532 nm.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 2.3-3.5%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 2.1-3.3%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896277950643,"sku":"E-BC-K814-M-48","price":190.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896277983411,"sku":"E-BC-K814-M-96","price":280.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"alpha-glucosidase-inhibitor-screening-kit-e-bc-d017-48","title":"Alpha-Glucosidase Inhibitor Screening Kit - E-BC-D017-48","description":"\u003cp\u003eAlpha-Glucosidase Inhibitor Screening Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-D017-48, E-BC-D017-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e \n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Glycolysis and Lipid Metabolism\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Inhibition rate\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Inhibitor\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 30 min\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: Alpha-glucosidase catalyzes the release of α-glucose from the non-reducing end of the substrate and promotes the absorption of glucose by the small intestine. Inhibition of α-glucosidase is an effective method for the treatment of non-insulin-dependent diabetes mellitus (NIDDM).\u003cbr\u003e\nThe detection principle of this kit: α-Glucosidase catalyzes the substrate reaction to generate colored products, whose absorbance increases at 400 nm. The addition of an inhibitor suppresses the enzymatic activity of α-glucosidase, resulting in a reduced rate of absorbance increase. The inhibition rate can be calculated based on the absorbance difference.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 5.6-8.6%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 0.2-2.0%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896284995763,"sku":"E-BC-D017-48","price":330.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896285028531,"sku":"E-BC-D017-96","price":490.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"mitochondrial-stress-fluorometric-assay-kit-e-bc-f078-48","title":"Mitochondrial Stress Fluorometric Assay Kit - E-BC-F078-48","description":"\u003cp\u003eMitochondrial Stress Fluorometric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-F078-48, E-BC-F078-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Glycolysis and Lipid Metabolism; Mitochondria Function\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Cell-based (quantitative)\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Fluorescence microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Fluorometric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Cell\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2 h 30 min\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: Mitochondrial stress refers to the stress state that occurs when mitochondria are damaged or overloaded under the influence of external or internal factors (such as oxidative stress, nutritional deficiency, toxins, etc.). This kind of stress can affect the energy production of mitochondria, leading to insufficient cellular energy and possibly triggering cell damage, inflammatory responses or promoting cell apoptosis. Long-term mitochondrial stress is closely related to various diseases (such as neurodegenerative diseases, cardiovascular diseases, metabolic diseases, etc.) and the aging process.\u003cbr\u003e\nThis kit provides an oxygen-sensitive fluorescent probe, which increases the fluorescence of the probe by the cells consuming oxygen. After adding the regulators oligomycin, carboxycyanide-4-trifluoromethoxylhydrazone (FCCP) and antimycin A, the effect of the metabolic process on maximum respiration can be visually presented.\u003cbr\u003e\nThe addition of oligomycin blocked ATP synthesis, slowed the electron transfer rate, reduced the respiratory rate and decreased oxygen consumption. FCCP is used to study the respiratory activity of mitochondria in the uncoupled state. After the uncoupled oxidative phosphorylation of FCCP, the proton pump of the electron transport chain (ETC) is no longer limited by ATP synthesis, the electron transport rate increases, and the oxygen consumption significantly increases, which is used to determine the maximum respiratory capacity. Antimycin A inhibits the main respiratory chain, and the electron transfer dependent on complex III stops, resulting in a significant decrease in basal oxygen consumption, which is used to measure the reserve respiratory rate.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896285061299,"sku":"E-BC-F078-48","price":350.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896285094067,"sku":"E-BC-F078-96","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"6-phosphogluconate-dehydrogenase-6-pgdh-activity-colorimetric-assay-kit-e-bc-k1150-m-48","title":"6-Phosphogluconate Dehydrogenase (6-PGDH) Activity Colorimetric Assay Kit - E-BC-K1150-M-48","description":"\u003cp\u003e6-Phosphogluconate Dehydrogenase (6-PGDH) Activity Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K1150-M-48, E-BC-K1150-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: 6-PGDH\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Glycolysis and Lipid Metabolism\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Enzyme activity\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.26-101.85 U\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum;Plasma;Animal and plant tissue;Cell\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 50 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 1.26 U\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: 6-phosphogluconate dehydrogenase (6-PGDH) is the rate-limiting enzyme of the pentose phosphate pathway, which is responsible for the production of NADPH, energy balance, growth rate, and cell viability. 6-PGDH is also associated with a variety of tumor signaling pathways, including epidermal growth factor signaling pathway and nuclear factor E2 related factor signaling pathway.\u003cbr\u003e\nThe principle of this kit is that the product generated by the 6-PGDH catalytic substrate reacts with the chromogenic agent to generate the chromogenic substance, which has the maximum absorption at 450 nm, and is calculated by measuring the OD value at 450 nm 6-PGDH enzyme activity.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 5.9-9.4%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 2.9-4.6%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896285126835,"sku":"E-BC-K1150-M-48","price":300.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896285159603,"sku":"E-BC-K1150-M-96","price":460.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"chymotrypsin-activity-colorimetric-assay-kit-e-bc-k845-m-48","title":"Chymotrypsin Activity Colorimetric Assay Kit - E-BC-K845-M-48","description":"\u003cp\u003eChymotrypsin Activity Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K845-M-48, E-BC-K845-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Amino Acids and Proteins\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Enzyme activity\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.028-1.01 U\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Tissue\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 1 h 10 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 0.028 U\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Chymotrypsin is a serine trypsin secreted by the pancreas, which is mainly used for the decomposition of proteins during digestion. It specifically hydrolyzes peptide bonds of aromatic amino acids (such as phenylalanine, tryptophan, and tyrosine) in proteins to generate smaller peptides or amino acids for further absorption by the body. In addition, chymotrypsin is widely used in protein analysis and research in the laboratory, especially for protein sequencing and structural analysis.\u003cbr\u003e\nThe detection principle of this kit is: The substrate was converted to a colored substance after chymotrypsin catalysis, and the OD value was detected at a wavelength of 405 nm, and the enzyme activity was calculated from the OD value of the standard.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 1.2-9.7%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 1.8-4.0%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896285192371,"sku":"E-BC-K845-M-48","price":280.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896285225139,"sku":"E-BC-K845-M-96","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"branched-chain-amino-acids-bcaa-colorimetric-assay-kit-e-bc-k847-m-48","title":"Branched Chain Amino Acids (BCAA) Colorimetric Assay Kit - E-BC-K847-M-48","description":"\u003cp\u003eBranched Chain Amino Acids (BCAA) Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K847-M-48, E-BC-K847-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: BCAA\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Amino Acids and Proteins\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.009-2 mmol\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum;Plasma;Animal and plant tissue;Cell\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 1 h 10 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 0.009 mmol\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Branched Chain Amino Acids (BCAA), including leucine, isoleucine and valine, are essential amino acids for the human body, which have the functions of muscle synthesis, anti-protein breakdown and stimulating insulin production.\u003cbr\u003e\nThe detection principle of this kit is: the branched chain amino acid under the catalysis of enzymes to generate the product of reaction with chromogenic agent generated color material, which has maximum absorption at 450 nm. The content of BCAA in samples was calculated by measuring OD value at 450 nm and standard curve.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 3.4-8.5%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 0.6-1.0%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896285257907,"sku":"E-BC-K847-M-48","price":280.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896285290675,"sku":"E-BC-K847-M-96","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"uricase-acitivity-colorimetric-assay-kit-e-bc-k896-m-48","title":"Uricase Acitivity Colorimetric Assay Kit - E-BC-K896-M-48","description":"\u003cp\u003eUricase Acitivity Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K896-M-48, E-BC-K896-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas:  Liver and Renal Function\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Enzyme activity\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.06-4.00 U\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Animal tissue;Plant tissue\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 50 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 0.06 U\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Uricase is an enzyme in the purine decomposition pathway, which can specifically catalyze the oxidation of uric acid into allantoin with higher solubility, thereby reducing the blood uric acid level.\u003cbr\u003e\nThe detection principle of this kit is: uricase can oxidize the substrate, and the substrate has the maximum absorption peak at 293 nm. The uricase activity was calculated by measuring the OD value reduction at 293 nm.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 6.7-8.8%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 2.6-3.4%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896285356211,"sku":"E-BC-K896-M-48","price":280.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896285388979,"sku":"E-BC-K896-M-96","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"asparaginase-asnase-activity-colorimetric-assay-kit-e-bc-k909-m-48","title":"Asparaginase (ASNase) Activity Colorimetric Assay Kit - E-BC-K909-M-48","description":"\u003cp\u003eAsparaginase (ASNase) Activity Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K909-M-48, E-BC-K909-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: ASNase\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Amino Acids and Proteins\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Enzyme activity\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.27-182.52 U\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum;Plasma\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 40 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 1.27 U\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Asparaginase (ASNase) is a proteolytic enzyme that was first discovered in guinea pigs and is not naturally produced in humans. Asparaginase has been widely used in the food industry, inhibiting the production of acrylamide carcinogens by hydrolyzing asparagine. Asparaginase is also used to treat various diseases due to its unique anti-tumor properties, such as acute lymphocytic leukemia, non-Hodgkin's lymphoma, leukemia meningitis, lung cancer and ovarian cancer and other tumor diseases.\u003cbr\u003e\nThe detection principle of this kit is as follows: asparaginase catalyzes the substrate to generate aspartic acid, and aspartic acid is hydrolyzed under the catalytic action of the enzyme. The amount of NADH consumed by the reaction is directly proportional to the activity of asparaginase. NADH is absorbed at a wavelength of 340 nm, and the activity of asparaginase can be calculated based on the change in absorbance value per unit time.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 7.1-8.2%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 2.2-4.7%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896285454515,"sku":"E-BC-K909-M-48","price":280.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896285487283,"sku":"E-BC-K909-M-96","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"alpha-amylase-inhibitor-screening-kit-e-bc-d018-48","title":"Alpha-Amylase Inhibitor Screening Kit - E-BC-D018-48","description":"\u003cp\u003eAlpha-Amylase Inhibitor Screening Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-D018-48, E-BC-D018-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e \n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Glycolysis and Lipid Metabolism\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Inhibition rate\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Inhibitor\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 40 min\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: α-Amylase in human body can not only act on the end of starch, but also act on the α-1,4 glucoside bond inside starch molecules. The degradation products are glucose, maltose and dextrin containing α-1,6 glucoside bond branch chain, which plays an important role in the digestion of polysaccharide compounds in food. Inhibition of α-amylase activity can not only reduce the digestion and absorption of starch, but also allow undigested and absorbed starch to enter the large intestine, promote gastrointestinal motility, and further reduce the occurrence of type 2 diabetes, which is an important drug target for the treatment of diabetes.\u003cbr\u003e\nThe detection principle of this kit: α-Amylase catalyzes the substrate reaction to generate colored products, whose absorbance increases at 405 nm. The addition of an inhibitor suppresses the activity of α-Amylase, resulting in a reduced rate of absorbance increase. The inhibition rate can be calculated based on the absorbance difference.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 7.2-10.0%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 0.4-1.3%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896297545907,"sku":"E-BC-D018-48","price":330.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896297578675,"sku":"E-BC-D018-96","price":490.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"cathepsin-d-catd-activity-fluorometric-assay-kit-e-bc-f052-48","title":"Cathepsin D (CatD) Activity Fluorometric Assay Kit - E-BC-F052-48","description":"\u003cp\u003eCathepsin D (CatD) Activity Fluorometric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-F052-48, E-BC-F052-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CatD\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Amino Acids and Proteins\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Inhibition rate\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.02-30.00 U\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Fluorescence microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Fluorometric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum;Plasma;Animal tissue;Cell\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 50 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 0.02 U\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Cathepsin D (CatD) is an important intracellular aspartic acid protease and is widely present in the biological world. Cathepsin D has multiple biological functions and is involved in intracellular protein degradation, apoptosis and autophagy, etc.\u003cbr\u003e\nThe detection principle of this kit: CatD hydrolyzes the substrate to generate the chromogenic substance. The activity of cathepsin D in the sample is calculated by measuring the changes in fluorescence values at the excitation wavelength of 328 nm and the emission wavelength of 393 nm\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 3.6-4.7%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 2.3-3.1%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896297644211,"sku":"E-BC-F052-48","price":330.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896297676979,"sku":"E-BC-F052-96","price":490.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"},{"product_id":"sialic-acid-sa-colorimetric-assay-kit-enzyme-method-e-bc-k813-m-48","title":"Sialic Acid (SA) Colorimetric Assay Kit (Enzyme Method) - E-BC-K813-M-48","description":"\u003cp\u003eSialic Acid (SA) Colorimetric Assay Kit (Enzyme Method)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K813-M-48, E-BC-K813-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eCategory: Cell Metabolism Assays\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: SA\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: This product can be stored at 2-8°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic Diseases\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.05-4.00 mmol\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum;Plasma;Saliva;Milk\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 30 min\u003c\/p\u003e \n\n\u003cp\u003eSensitivity: 0.05 mmol\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Sialic acid (SA) is widely present in animals and microorganisms, usually in the form of oligosaccharides, glycolipids or glycoproteins, and is an important component of cell membranes. SA is located at the ends of cell membrane glycoproteins and glycolipids, and participates in various physiological functions such as cell recognition, adhesion and contact inhibition. It is a broad-spectrum tumor marker. When normal cells transform into malignant tumor cells, the structure and content of glycoproteins and glycolipids on the cell surface undergo significant changes. SA falls off and enters the blood, increasing the content of SA in serum. Measuring its level is of great significance for the auxiliary diagnosis, therapeutic effect observation and prognosis judgment of malignant tumors.\u003cbr\u003e\nSA is converted into pyruvic acid under the action of a series of enzymes. Pyruvic acid is converted into lactic acid and NAD+ under the action of lactate dehydrogenase and NADH. The concentration of SA in the sample can be indirectly measured by measuring the decrease in the absorbance of NADH at 340 nm.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 6.3-7.3%\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 3.2-4.5%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"48T","offer_id":45896297808051,"sku":"E-BC-K813-M-48","price":420.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45896297840819,"sku":"E-BC-K813-M-96","price":640.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Elabscience-logo-for-products.jpg?v=1787577484"}],"url":"https:\/\/afsbio.com\/collections\/cell-metabolism-assays.oembed","provider":"AFSBio Inc.","version":"1.0","type":"link"}