{"title":"ELISA, Best Selling ELISAs","description":null,"products":[{"product_id":"elisa-kit-for-anti-interferon-gamma-antibody-anti-ifng-aea049hu","title":"ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg) - AEA049Hu","description":"\u003cp\u003eELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)\u003c\/p\u003e\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\u003cp\u003eCatalogue Numbers: AEA049Hu-24, AEA049Hu-48, AEA049Hu-96\u003c\/p\u003e\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\u003cp\u003eFeatured Series Function: Detects protein (antibody)\u003c\/p\u003e\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\u003cp\u003eTest Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Interferon Gamma Antibody (Anti-IFNg) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Interferon Gamma Antibody (Anti-IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003eReactivity: Human\u003c\/p\u003e\u003cp\u003eAlternate Names: IFN-G, IFG, IFI, INFr, IFN, Immune Interferon\u003c\/p\u003e\u003cp\u003eTarget: Interferon Gamma,\u003c\/p\u003e\u003cp\u003eAssay Length: 2.5h\u003c\/p\u003e\u003cp\u003eSample Size: 50uL\u003c\/p\u003e\u003cp\u003eSample Type: Serum\u003c\/p\u003e\u003cp\u003eDetection Method: Competitive Inhibition\u003c\/p\u003e\u003cp\u003eDetection Range: 3.12-200ng\/mL\u003c\/p\u003e\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD\/meanX100\u003c\/p\u003e\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\u003cp\u003eAssay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 5 times; 5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 6. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\u003cp\u003eSpecificity: Reactive with Human IFNg \/ Interferon Gamma\u003c\/p\u003e\u003cp\u003eSensitivity: 1.22ng\/mL\u003c\/p\u003e\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\u003cp\u003eShelf Life: 12 months\u003c\/p\u003e\u003cp\u003eGene ID: 3458\u003c\/p\u003e\u003cp\u003eUniProt: P01579\u003c\/p\u003e\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud Clone Corp","offers":[{"title":"24T","offer_id":45379598221491,"sku":"AEA049Hu-24","price":360.0,"currency_code":"USD","in_stock":true},{"title":"48T","offer_id":45379598254259,"sku":"AEA049Hu-48","price":630.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45379598287027,"sku":"AEA049Hu-96","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/CCC_Image.png?v=1790956630"},{"product_id":"elisa-kit-for-anti-tumor-necrosis-factor-alpha-antibody-anti-tnfa-aea133hu","title":"ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) - AEA133Hu","description":"\u003cp\u003eELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)\u003c\/p\u003e\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\u003cp\u003eCatalogue Numbers: AEA133Hu-24, AEA133Hu-48, AEA133Hu-96\u003c\/p\u003e\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\u003cp\u003eFeatured Series Function: Detects protein (antibody)\u003c\/p\u003e\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\u003cp\u003eTest Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003eReactivity: Human\u003c\/p\u003e\u003cp\u003eAlternate Names: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2\u003c\/p\u003e\u003cp\u003eTarget: Tumor Necrosis Factor Alpha,\u003c\/p\u003e\u003cp\u003eAssay Length: 2.5h\u003c\/p\u003e\u003cp\u003eSample Size: 50uL\u003c\/p\u003e\u003cp\u003eSample Type: Serum, Plasma, Other Biological Fluids\u003c\/p\u003e\u003cp\u003eDetection Method: Competitive Inhibition\u003c\/p\u003e\u003cp\u003eDetection Range: 3.12-200ng\/mL\u003c\/p\u003e\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD\/meanX100\u003c\/p\u003e\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\u003cp\u003eAssay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 5 times; 5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 6. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\u003cp\u003eSpecificity: Reactive with Human TNFa \/ Tumor Necrosis Factor Alpha\u003c\/p\u003e\u003cp\u003eSensitivity: 1.13ng\/mL\u003c\/p\u003e\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\u003cp\u003eShelf Life: 12 months\u003c\/p\u003e\u003cp\u003eGene ID: 7124\u003c\/p\u003e\u003cp\u003eUniProt: P01375\u003c\/p\u003e\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud Clone Corp","offers":[{"title":"24T","offer_id":45379599073459,"sku":"AEA133Hu-24","price":360.0,"currency_code":"USD","in_stock":true},{"title":"48T","offer_id":45379599106227,"sku":"AEA133Hu-48","price":630.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45379599138995,"sku":"AEA133Hu-96","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/CCC_Image.png?v=1790956630"}],"url":"https:\/\/afsbio.com\/collections\/elisa-best-selling-elisas.oembed","provider":"AFSBio Inc.","version":"1.0","type":"link"}