A label-free high-content system
Livecyte changes what's possible to measure using live cell assays
- Livecyte produces exceptionally high contrast time-lapse videos using Phasefocus’s patented Ptychographic quantitive phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.
- Automated single-cell tracking of even the most sensitive cells quickly reveals subtle phenotypic differences in unperturbed cell populations.
- Easy-to-use Dashboards present coherent and concise results from up to 96 wells at a time whilst retaining the ability to investigate individual cell behaviour and outlying characteristics.
Applications in:
Cell Growth and Proliferation
- Livecyte measures individual cell growth independently from proliferation.
- Cell growth is more complex than just cells multiplying
- They can increase in size without dividing, they can divide asymmetrically, they can grow to a certain size then stop. All these aspects of cell growth are lost with most live cell assays. Not with Livecyte.
Measure true cell growth at the single cell level!
Wound Healing
- Directly measure cell motility with individual cells
- Separate out migration from proliferation in a single experiment
- Observe differences between leading edge and following cells
- Correlate motility to morphological differences
Go beyond traditional wound healing assays
Cell Motility
- Livecyte automatically tracks cells, even in a 96-well plate.
- Just seed your cells, monitor with Livecyte and a full range of cell migration parameters will be generated.
- Compare the speed, directionality, confinement and heterogeneity of different populations.
Automatically measure cell motility without a scratch wound assay
Oncology
- Directly measure mitotic time with individual cells
- Measure random and chemotactic migration
- Identify and characterise differences in subpopulations in heterogenous primary cultures
- Correlate motility to morphological differences
- Generate statistically valid results from multiple repeats in 96-wells
Automatically measure the morphology and motion of every cell
Toxicology
- True label-free cell count, not just confluence
- Monitor dry mass to automatically determine viability and cell death
- Statistically robust results from multiple repeats in 96-well plates
- Generate label-free dose-response curves
- Identify dynamic toxic responses missed by other methods
Go beyond confluence-based toxicity assays
Angiogenesis
- Label-free timelapse tube formation assays
- High-resolution large fields-of-view with no stitching
- Perform assays on Matrigel and standard plates
- Automated post-acquisition focus ensures consistent image quality for every well
- Simple, yet powerful analysis of tube formation metrics with Application Dashboard outputs
Automated analysis to determine tube length, formation, diameter etc
Stem Cells
- Identify morphological phenotypes
- Watch and quantify cell differentiation
- Monitor stem cell populations, for many days, without perturbing cell behaviour
- Image through plastic and coated plates
- Image on extracellular matrices
- Cells are still viable after imaging
Obtain quantitative measurements without perturbing your cells
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