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Quantitative Label-Free Live Cell Imaging

Phasefocus Livecyte: high content live-cell assays from 96 wells to single cells

LiveCyte 2 - Let Every Cell Tell its Story

Stop manually tracking your cells; let Livecyte do it for you automatically.

Now you can follow every individual cell, measuring how they move and change in response to changing culture conditions.

Livecyte™ identifies up to 19 different morphological characteristics for each cell, generating a unique phenotypic fingerprint by which thousands of individual cells can be automatically tracked, even within heterogeneous cell populations.

The result is a phenomenal new depth of information, compared to HCA or standard time-lapse instruments.

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Cell Growth and Proliferation

- Livecyte measures individual cell growth independently from proliferation.
- Cell growth is more complex than just cells multiplying
- They can increase in size without dividing, they can divide asymmetrically, they can grow to a certain size then stop. All these aspects of cell growth are lost with most live cell assays. Not with Livecyte.

Measure true cell growth at the single cell level

Wound Healing

- Directly measure cell motility with individual cells
- Separate out migration from proliferation in a single experiment
- Observe differences between leading edge and following cells
- Correlate motility to morphological differences

Go beyond traditional wound healing assays

Cell Motility

- Livecyte automatically tracks cells, even in a 96-well plate.
- Just seed your cells, monitor with Livecyte and a full range of cell migration parameters will be generated.
- Compare the speed, directionality, confinement and heterogeneity of different populations.

Automatically measure cell motility without a scratch wound assay


- Directly measure mitotic time with individual cells
- Measure random and chemotactic migration
- Identify and characterise differences in subpopulations in heterogenous primary cultures
- Correlate motility to morphological differences
- Generate statistically valid results from multiple repeats in 96-wells.

Automatically measure the morphology and motion of every cell


- True label-free cell count, not just confluence
- Monitor dry mass to automatically determine viability and cell death
- Statistically robust results from multiple repeats in 96-well plates
- Generate label-free dose-response curves
Identify dynamic toxic responses missed by other methods

Go beyond confluence-based toxicity assays


- Label-free timelapse tube formation assays
- High-resolution large fields-of-view with no stitching
- Perform assays on Matrigel and standard plates
- Automated post-acquisition focus ensures consistent image quality for every well
- Simple, yet powerful analysis of tube formation metrics with Application Dashboard outputs

Automated analysis to determine tube length, formation, diameter etc

Stem Cells

- Identify morphological phenotypes
- Watch and quantify cell differentiation
- Monitor stem cell populations, for many days, without perturbing cell behaviour
- Image through plastic and coated plates
- Image on extracellular matrices
- Cells are still viable after imaging

Obtain quantitative measurements without perturbing your cells 

Mitotic Time
Full Wound Healing
Multi-panel Video


- Fully automated Brightfield, Ptychographic QPI and Fluorescence modalities.
- 6 position objective turret — range of objectives available.
- Lateral Resolution — 0.4 µm.
- Axial Resolution — Typically 40 nm.
- Illumination Power QPI — 0.4uWmm2.
- Detector — sCMOS air cooled (2000X2000). Pix Size—6.5 µm.


- Temperature controlled macro-environment (adjustable from 28 oC—40 oC).

- Micro-environment controlling CO2, humidity and evaporation.


- Design Module.
- Acquire Module.
- Analyse Module.
- Application Specific Dashboards.


- Acquisition PC.
- PiBOX (reconstruct QPI data).
- CatBOX (analysis of data).
- NAS (Storage solution offering 12 TB of data—optional upgrades available).


- Bench space required: 1600 mm X 800 mm.
- Height above bench: 750 mm.
- Under bench space for rack: 700 mm X 600 mm X 800 mm.

No specialised optical table (anti-vibration) is required.

Application Notes
Technical Notes
Why is the LiveCyte system a new way of looking at my cells?
What technique does LiveCyte use to obtain phase information?
Why is LiveCyte different to traditional photocytometers?
Why is the Livecyte different to traditional light microscopes?
In particular, how does the Livecyte images differ from phase contrast images?
How does Livecyte support continuous non-invasive imaging and analysis?
How long can cells be imaged for?
What do you mean when you say the images are quantitative?
How is the dry mass measurement defined on Livecyte?
How is wound closure quantified?
How do I create a uniform and consistent scratch on the plate to assess cell motility for scratch wound analysis?
What is the significance of being able to track leader cells and the cells further back in the scratch wound?
Why do you refer to the system as efficient?
Why does the system also have fluorescence capabilities?
What is the resolution of the images produced?
What is the biggest Field of View (FOV) that you can image and does it always have to be square?
What is the nature of the data produced?
What comes as standard with the system?
What well plates and coatings can I use on Livecyte?
What are some example applications for LiveCyte?
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A label-free high-content system 

Livecyte changes what's possible to measure using live cell assays 

- Livecyte produces exceptionally high contrast time-lapse videos using Phasefocus’s patented Ptychographic quantitive phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.

- Automated single-cell tracking of even the most sensitive cells quickly reveals subtle phenotypic differences in unperturbed cell populations.

- Easy-to-use dashboards present coherent and concise results from up to 96 wells at a time whilst retaining the ability to investigate individual cell behaviour and outlying characteristics.

High Contrast Without Compromise 

Fluorescence-like images without the compromise.

Livecyte's label-free imaging eliminates the constraints of phototoxicity and population averaged analysis, providing a more accurate and realistic account of treatment driven changes in cell behaviour.

Avoid the fluorescence paradox of added cost and perturbed cells. Livecyte easily produces high resolution, high contrast images from which individual cells can be readily defined and tracked for prolonged periods.

Powerful Integrated Analysis

- Integrated analysis suite enables cell behaviour to be monitored as a function of treatment

- Easily export results in Excel and Graphpad formats

- Monitor many different types of behaviour, from morphological to motile, in every experiment

- No need for multiple separate experiments

From assay level....

Down to single cell level...

Livecyte is a True Assay-Driven Tool

No Calibration, No Dedicated Consumables, No Hidden Costs. 

Good science requires multiple repeats and comparisons across several treaments.
Using multi-well plates is the only way to do this efficiently and cost-effectively.

Livecyte's unique meniscus compensation and perfect focus technologies make it simple to automatically perform robust label-free assays within any standard plate, up to 96 wells.

Please contact us to learn more! 

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Award-winning Technology

Livecyte's patented technology uses a quantitative phase imaging (QPI) technique known as Ptychography. Phasefocus was awarded a Microscope Today Innovation Award in 2013 for the technology, and won a second award in 2017 for Livecyte itself.
In 2018, researchers from Cornell University set the Guinness World Record for the highest resolution microscope using Ptychography on an Electron Microscope.

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