{"product_id":"14-3-3-β-q69-polyclonal-antibody-bs2176","title":"14-3-3 β (Q69) Polyclonal Antibody -BS2176","description":"\u003cp\u003e14-3-3 β (Q69) Polyclonal Antibody\u003c\/p\u003e \n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2176-50, BS2176-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P31946\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine\/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import\/export sequences, prevention of degradation, activation\/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: 14-3-3 β (Q69) polyclonal antibody detects endogenous levels of 14-3-3 β protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of 14-3-3 β (Q69) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane 1: U-87MG whole cell lysate (40ug)\u003cbr\u003e\nLane 2: MCF-7 whole cell lysate (40ug)\u003cbr\u003e\nLane 3: K562 whole cell lysate (40ug)\u003cbr\u003e\nLane 4: HepG2 whole cell lysate (40ug)\u003cbr\u003e\nLane 5: HEK293T whole cell lysate (40ug)\u003cbr\u003e\nLane 6: PC12 whole cell lysate (40ug)\u003cbr\u003e\nLane 7: CT-26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of 14-3-3 β (Q69) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 28 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 14-3-3 protein beta\/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; YWHAB; 14-3-3β; 14-3-3 α; 14-3-3α; 1433B; 14-3-3 β\/α; 14-3-3β\/α;\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 40-90 of Human 14-3-3 β.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953220849843,"sku":"BS2176-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953220915379,"sku":"BS2176-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_69364d9a-df72-4d25-80b1-5eefbbd7a07d.jpg?v=1705057029","url":"https:\/\/afsbio.com\/products\/14-3-3-%ce%b2-q69-polyclonal-antibody-bs2176","provider":"AFSBio Inc.","version":"1.0","type":"link"}