{"product_id":"14-3-3-ζ-a79-polyclonal-antibody-bs2385","title":"14-3-3 ζ (A79) Polyclonal Antibody-BS2385","description":"\u003cp\u003e14-3-3 ζ (A79) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2385-50, BS2385-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P63104\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine\/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import\/export sequences, prevention of degradation, activation\/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: 14-3-3 ζ (A79) polyclonal antibody detects endogenous levels of 14-3-3 protein zeta\/delta.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 28 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of 14-3-3 ζ (A79) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: PC3 whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: A549 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: K562 whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: C6 whole cell lysate (40ug)\u003cbr\u003e\n Lane 5: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (A79) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (A79) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (A79) pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 14-3-3 protein zeta\/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; 14-3-3 protein zeta; 14-3-3 protein delta; 14-3-3 ζ; 14-3-3 δ; 1433Z; KCIP1; KCIP 1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 49-100 of Human 14-3-3 ζ.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044056826035,"sku":"BS2385-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044056858803,"sku":"BS2385-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_74ce2db6-7529-440b-b267-1337da906c3b.jpg?v=1707739204","url":"https:\/\/afsbio.com\/products\/14-3-3-%ce%b6-a79-polyclonal-antibody-bs2385","provider":"AFSBio Inc.","version":"1.0","type":"link"}