{"product_id":"14-3-3-θ-q67-polyclonal-antibody-bs2179","title":"14-3-3 θ (Q67) Polyclonal Antibody-BS2179","description":"\u003cp\u003e14-3-3 θ (Q67) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2179-50, BS2179-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P27348\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau\/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau\/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau\/theta also binds BCR\/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: 14-3-3 θ (Q67) polyclonal antibody detects endogenous levels of 14-3-3 protein theta\/tau.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 28 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of 14-3-3 θ (Q67) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: K562 whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: HepG2 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: A549 whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: HEK293T whole cell lysate (40ug)\u003cbr\u003e\n Lane 5: U-87MG whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of 14-3-3 θ (Q67) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 θ (Q67) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 θ (Q67) pAb in paraffin-embedded human lung carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAQ; 14-3-3 θ; 14-3-3 τ; 1433T\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human 14-3-3 θ.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044056563891,"sku":"BS2179-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044056596659,"sku":"BS2179-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e2536f34-768c-4c7c-a15b-e8328e7c073e.jpg?v=1707739197","url":"https:\/\/afsbio.com\/products\/14-3-3-%ce%b8-q67-polyclonal-antibody-bs2179","provider":"AFSBio Inc.","version":"1.0","type":"link"}