{"product_id":"14-3-3-θ-w228-polyclonal-antibody-bs1790","title":"14-3-3 θ (W228) Polyclonal Antibody-BS1790","description":"\u003cp\u003e14-3-3 θ (W228) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1790-50, BS1790-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P27348\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau\/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau\/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau\/theta also binds BCR\/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: 14-3-3 θ (W228) polyclonal antibody detects endogenous levels of 14-3-3 protein theta\/tau.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 28 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of 14-3-3 θ (W228) polyclonal antibody at 1:500 dilution \u003cbr\u003e\n Lane 1: Hela whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: C6 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: NIH-3T3 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of 14-3-3 θ (W228) pAb in paraffin-embedded human breast carcinoma tissue at 1:50. showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 θ (W228) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., IF image of BS1790 stained A375 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody 14-3-3 θ (W228) pAb #BS1790(1:100) at 10µg\/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Mouse IgG (H+L) FITC#BS10950 used at a 1\/400 dilution for 2h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAQ; 14-3-3 θ; 14-3-3 τ; 1433T\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 200-240 of Human 14-3-3 θ.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044055744691,"sku":"BS1790-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044055777459,"sku":"BS1790-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_6a84e891-6be8-48d4-a3d4-3440b60f8ce3.jpg?v=1707739179","url":"https:\/\/afsbio.com\/products\/14-3-3-%ce%b8-w228-polyclonal-antibody-bs1790","provider":"AFSBio Inc.","version":"1.0","type":"link"}