{"product_id":"4e-bp1-phospho-thr70-polyclonal-antibody-ap0155","title":"4E-BP1 (Phospho-Thr70) Polyclonal Antibody - AP0155","description":"\u003cp\u003e4E-BP1 (Phospho-Thr70) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0155-50, AP0155-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and resμlts in activation of cap-dependent translation. Both the PI3 kinase\/Akt pathway and FRAP\/mTOR kinase regμlate 4E-BP1 activity. Mμltiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP\/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regμlated by insμlin 1, PHAS-I, EIF4EBP1\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Threonine 70.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:1000~1:2000\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: 4E-BP1(Phospho-Thr70) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr70.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 18 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: PI3K AKT signaling, Hypoxia Signaling, Insμlin \u0026amp;Glucose Signaling, MTOR signaling,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: Q13541\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946163527859,"sku":"AP0155-50","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946163560627,"sku":"AP0155-100","price":492.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/4e-bp1-phospho-thr70-polyclonal-antibody-ap0155","provider":"AFSBio Inc.","version":"1.0","type":"link"}