{"product_id":"4ebp1-phospho-t46-rabbit-monoclonal-antibody-mb66300","title":"4EBP1 (Phospho-T46) Rabbit Monoclonal Antibody - MB66300","description":"\u003cp\u003e4EBP1 (Phospho-T46) Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66300-50, MB66300-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and resμlts in activation of cap-dependent translation. Both the PI3 kinase\/Akt pathway and FRAP\/mTOR kinase regμlate 4E-BP1 activity. Mμltiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP\/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IP\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regμlated by insμlin 1, PHAS-I\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr45 of human eIF4EBP1\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/500 - 1\/1000), IHC (1\/50 - 1\/100), IP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of 4EBP1 (pT46) protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 15 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: AMPK Signaling Pathway, Tumor Angiogenesis, Insμlin \u0026amp;Glucose Signaling, PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase, MTOR signaling\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: Q13541\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946399195315,"sku":"MB66300-50","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946399228083,"sku":"MB66300-100","price":492.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/4ebp1-phospho-t46-rabbit-monoclonal-antibody-mb66300","provider":"AFSBio Inc.","version":"1.0","type":"link"}