{"product_id":"a-raf-polyclonal-antibody-bs90082","title":"A-RAF Polyclonal Antibody-BS90082","description":"\u003cp\u003eA-RAF Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90082-50, BS90082-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P10398(Human)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Several serine\/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK\/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine\/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine\/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: A-RAF polyclonal antibody detects endogenous levels of A-RAF protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 68 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of A-RAF on Hela cells lysates using anti-A-RAF antibody at 1\/1,000 dilution., ICC staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ARAF, A raf 1, A Raf proto oncogene serine\/threonine protein kinase, ARAF 1, Araf, ARaf proto oncogene serine\/threonine protein kinase, ARAF, ARAF1, Oncogene Araf1, Oncogene PKS2, PKS 2, PKS, PKS2, Proto oncogene Pks, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, Serine\/threonine-protein kinase A-Raf, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 (murine sarcoma 3611 virus),\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979760767155,"sku":"BS90082-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979760799923,"sku":"BS90082-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ce8d6069-ecc0-4879-a4fa-537d12033bb2.jpg?v=1705748789","url":"https:\/\/afsbio.com\/products\/a-raf-polyclonal-antibody-bs90082","provider":"AFSBio Inc.","version":"1.0","type":"link"}