{"product_id":"acsl1-polyclonal-antibody-bs76706","title":"ACSL1 Polyclonal Antibody-BS76706","description":"\u003cp\u003eACSL1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS76706-50, BS76706-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P33121\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200   |   IP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 78KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using ACSL1 antibody at 1:1000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 10s., Immunofluorescence analysis of C6 cells using ACSL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH-3T3 cells using ACSL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug ACSL1 antibody. Western blot was performed from the immunoprecipitate using ACSL1 antibody at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ACS1; FACL1; FACL2; LACS; LACS1; LACS2; ACSL1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human ACSL1(NP_001986.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047639810227,"sku":"BS76706-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047639842995,"sku":"BS76706-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_bec0127b-7cfe-440d-ae20-291ae5187c40.jpg?v=1707826567","url":"https:\/\/afsbio.com\/products\/acsl1-polyclonal-antibody-bs76706","provider":"AFSBio Inc.","version":"1.0","type":"link"}