{"product_id":"actinin-α2-3-q58-polyclonal-antibody-bs1984","title":"Actinin-α2\/3 (Q58) Polyclonal Antibody-BS1984","description":"\u003cp\u003eActinin-α2\/3 (Q58) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1984-50, BS1984-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35609\/Q08043\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Actinin-α2\/3 (Q58) polyclonal antibody detects endogenous levels of actinin-α2 and actinin-α3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 103 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Actinin-α2\/3 (Q58) polyclonal antibody at 1:500 dilution\u003cbr\u003e\n Lane 1: Hela cell lysate\u003cbr\u003e\n Lane 2: Mouse kidney tissue lysate\u003cbr\u003e\n Lane 3: H9C2 cell lysate, Immunohistochemistry (IHC) analyzes of Actinin-α2\/3 (Q58) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:5000. Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Actinin-α2\/3 (Q58) pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase, Immunohistochemistry (IHC) analyzes of Actinin-α2\/3 (Q58) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2; α-Actinin2; Actinin α2; Actininα2; αActinin-2; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; α-Actinin3; Actinin α3; Actininα3; αActinin-3; Actinin-α3; α-actinin-2; α-actinin-3\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 30-70 of Human Actinin-α2.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044056039603,"sku":"BS1984-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044056072371,"sku":"BS1984-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_486be1b1-6abd-4f6f-ae6a-014485e3b285.jpg?v=1707739186","url":"https:\/\/afsbio.com\/products\/actinin-%ce%b12-3-q58-polyclonal-antibody-bs1984","provider":"AFSBio Inc.","version":"1.0","type":"link"}