{"product_id":"ahr-phospho-s36-polyclonal-antibody-ap0302","title":"AhR (phospho-S36) Polyclonal Antibody-AP0302","description":"\u003cp\u003eAhR (phospho-S36) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0302-50, AP0302-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35869\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix\/loop\/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).Unlike the steroid\/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-AhR (S36) polyclonal antibody detects endogenous levels of AhR only when phosphorylated at Ser36.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 94 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-AhR (S36) at 1:2000 dilution\u003cbr\u003e\n Lane 1: MCF-7 whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: The Heart tissue lysate of Mouse (40ug)\u003cbr\u003e\n Lane 3: The Heart tissue lysate of Rat (40ug)\u003cbr\u003e\n Lane 4: HepG2 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of AhR (phospho-S36) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of AhR (phospho-S36) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR; BHLHE76\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human AhR around the phosphorylation site of Serine 36.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993818894515,"sku":"AP0302-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993818927283,"sku":"AP0302-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_306be92b-8e14-4387-a3f9-053eaf17e778.jpg?v=1706183915","url":"https:\/\/afsbio.com\/products\/ahr-phospho-s36-polyclonal-antibody-ap0302","provider":"AFSBio Inc.","version":"1.0","type":"link"}