{"product_id":"alas1-rabbit-monoclonal-antibody-bs9823m","title":"ALAS1 Rabbit Monoclonal Antibody-BS9823M","description":"\u003cp\u003eALAS1 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9823M-50, BS9823M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P13196\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:5000\u003cbr\u003e\nICC\/IF: 1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of ALAS and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 71 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of ALAS1 Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: JAR whole cell lysate\u003cbr\u003e\n Lane 2: HUVEC whole cell lysate\u003cbr\u003e\n Lane 3: Hela whole cell lysate, ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue).\u003cbr\u003e\n Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 5-aminolevulinate synthase, nonspecific, mitochondrial; ALAS-H; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1; ALAS1; ALAS3; ALASH; OK\/SW-cl.121\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989567279283,"sku":"BS9823M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989567312051,"sku":"BS9823M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_bb216013-bcf0-4493-859e-1a0fa894fa87.jpg?v=1706095498","url":"https:\/\/afsbio.com\/products\/alas1-rabbit-monoclonal-antibody-bs9823m","provider":"AFSBio Inc.","version":"1.0","type":"link"}