{"product_id":"alox15-polyclonal-antibody-bs80489","title":"ALOX15 Polyclonal Antibody-BS80489","description":"\u003cp\u003eALOX15 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS80489-50, BS80489-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P16050\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: IHC,1:50 - 1:200   |   IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. It inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z-eicosatetraenoate producing both 12-hydroperoxyeicosatetraenoate\/12-HPETE and 15-hydroperoxyeicosatetraenoate\/15-HPETE. It may then act on 12-HPETE to produce hepoxilins, which may show proinflammatory properties (By similarity. Can also peroxidize linoleate ((9Z,12Z-octadecadienoate to 13-hydroperoxyoctadecadienoate\/13-HPODE. May participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoate to generate specialized pro-resolving mediators (SPMslike resolvin D5 ((7S,17S-diHPDHA and (7S,14S-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets. Can convert epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution leading to the formation of monocyclic endoperoxides. Plays an important role during the maintenance of self-tolerance by peroxidizing membrane-bound phosphatidylethanolamine which can then signal the sorting process for clearance of apoptotic cells during inflammation and prevent an autoimmune response.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunohistochemistry of paraffin-embedded mouse intestin using ALOX15 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spleen using ALOX15 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat spleen using ALOX15 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of MCF7 cells using ALOX15 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ALOX15; 12-LOX; 15-LOX-1; 15LOX-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human ALOX15(NP_001131.3).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43050219733171,"sku":"BS80489-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43050219765939,"sku":"BS80489-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2254507b-3f0b-445d-9473-f6043dd07038.jpg?v=1707913473","url":"https:\/\/afsbio.com\/products\/alox15-polyclonal-antibody-bs80489","provider":"AFSBio Inc.","version":"1.0","type":"link"}