{"product_id":"alx3-r203-polyclonal-antibody-bs1898","title":"ALX3 (R203) Polyclonal Antibody-BS1898","description":"\u003cp\u003eALX3 (R203) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1898-50, BS1898-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O95076\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: Aristaless-related genes are a group of paired-related homeobox genes which play a role in regulating vertebrate embryogenesis. The homeodomain transciption factor aristaless-like 3 (ALX3) is expressed in mouse embryos from 8 days of gestation, predominantly in neural crest-derived mesenchyme and in lateral plate mesoderm. Expression analysis of human and mouse tissue reveals predominant ALX3 expression in brain tissue. The Alx3 gene maps to chromosome 1p23-p13 and encodes a 343 amino acid protein. Preferential methylation of Alx3 occurs in advanced-stage neuroblastoma and may repress ALX3 expression. Treatment with the methylation inhibitor 5-aza-2’-deoxycytidine restores ALX3 expression. Alx3 (-) mice lack a phenotype distinct from wild-type mice, however Alx3\/Alx4 double mutants demonstrate severe craniofacial abnormalities not present in Alx4 single mutants. Specifically, Alx3\/Alx4 double mutant newborn mice have cleft nasal regions in addition to malformation of other neural crest-derived skull structures.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: ALX3 (R203) polyclonal antibody detects endogenous levels of ALX3 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of ALX3 (R203) pAb at 1:1000 dilution\u003cbr\u003e\nLane 1: HepG2 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: Hela whole cell lysate (40ug)\u003cbr\u003e\nLane 3: MCF-7 whole cell lysate (40ug)\u003cbr\u003e\nLane 4: PMVEC whole cell lysate (40ug)\u003cbr\u003e\nLane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ALX3 (R203) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 36 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Homeobox protein aristaless-like 3; Proline-rich transcription factor ALX3;\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human ALX3.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953215967411,"sku":"BS1898-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953216000179,"sku":"BS1898-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_990cb1ba-9eee-4e06-b9fc-92ef0f17bbbd.jpg?v=1705056935","url":"https:\/\/afsbio.com\/products\/alx3-r203-polyclonal-antibody-bs1898","provider":"AFSBio Inc.","version":"1.0","type":"link"}