{"product_id":"ampkα1-2-phospho-thr183-172-rabbit-polyclonal-antibody-es1451","title":"AMPKα1\/2 (phospho Thr183\/172) Rabbit Polyclonal Antibody - ES1451","description":"\u003cp\u003eAMPKα1\/2 (phospho Thr183\/172) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1451-50, ES1451-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: approximately 7-10 business days, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene belongs to the ser\/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP\/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],\u003c\/p\u003e \n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Monkey, Pig\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: IF, WB, IHC, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: AMPKα1\/2,\u003c\/p\u003e \n\n\u003cp\u003eSpecificity: Phospho-AMPKα1\/2 (T183\/172) Polyclonal Antibody detects endogenous levels of AMPKα1\/2 protein only when phosphorylated at T183\/172.\u003c\/p\u003e\n\n\u003cp\u003eDilution: IF: 1:50-200 Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/40000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e \n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Brain, Intestine, Liver, Mammary gland, Platelet, Testis\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 63kD\u003c\/p\u003e\n\n\u003cp\u003eFields: FoxO signaling pathway, Autophagy - animal, mTOR signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Longevity regulating pathway, Longevity regulating pathway - multiple species, Apelin signaling pathway, Tight junction, Circadian rhythm, Thermogenesis, Insulin signaling pathway, Adipocytokine signaling pathway, Oxytocin signaling pathway, Glucagon signaling pathway, Insulin resistance, Non-alcoholic fatty liver disease, Alcoholic liver disease, Hypertrophic cardiomyopathy, Fluid shear stress and atherosclerosis\u003c\/p\u003e\n\n\u003cp\u003eFunction: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP\/ATP ratio. Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and\/or hypoxia. This is a catalytic s\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm Nucleus In response to stress, recruited by p53\/TP53 to specific promoters.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: AAPK1\/AAPK2\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1\/2\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 5562\/5563\u003c\/p\u003e\n\n\u003cp\u003eHuman SwissProt Number: Q13131\/P54646\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 105787\/108079\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 65248\/78975\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: P54645\/Q09137\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":45825501233331,"sku":"ES1451-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45825501266099,"sku":"ES1451-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk-biotechnology-logo-product.jpg?v=1786476023","url":"https:\/\/afsbio.com\/products\/ampk%ce%b11-2-phospho-thr183-172-rabbit-polyclonal-antibody-es1451","provider":"AFSBio Inc.","version":"1.0","type":"link"}