{"product_id":"ampkα1-polyclonal-antibody-bs80110","title":"AMPKα1 Polyclonal Antibody-BS80110","description":"AMPKα1 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS80110-50, BS80110-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: Q13131\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IHC\n\nAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200\n\nBackground: The protein encoded by this gene belongs to the ser\/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP\/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 64kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using AMPKα1 alpha 1 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Enhanced Kit.\n Exposure time: 3min., Immunohistochemistry of paraffin-embedded rat spleen using AMPKα1 alpha 1 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human tonsil using AMPKα1 alpha 1 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.\n\nAlternative Name: PRKAA1; AMPK; AMPKa1\n\nImmunogen: A synthetic peptide of human AMPKα1(NP_006242.5).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997550448819,"sku":"BS80110-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997550481587,"sku":"BS80110-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_fb876399-9e52-4ca1-880f-bf18e214f1d2.jpg?v=1706270317","url":"https:\/\/afsbio.com\/products\/ampk%ce%b11-polyclonal-antibody-bs80110","provider":"AFSBio Inc.","version":"1.0","type":"link"}