{"product_id":"ampkα2-polyclonal-antibody-bs75574","title":"AMPKα2 Polyclonal Antibody-BS75574","description":"\u003cp\u003eAMPKα2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS75574-50, BS75574-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P54646\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 62kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of various lysates, using PRKAA2 antibody at 1:920 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 10s., Immunofluorescence analysis of NIH\/3T3 cells using [KO Validated] AMPKα2 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using [KO Validated] AMPKα2 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using [KO Validated] AMPKα2 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: PRKAA2; AMPK; AMPK2; AMPKa2; PRKAA\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human AMPKα2(NP_006243.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047630733491,"sku":"BS75574-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047630766259,"sku":"BS75574-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c8bda2c6-c53c-4355-a3c6-aeabc3d768da.jpg?v=1707826307","url":"https:\/\/afsbio.com\/products\/ampk%ce%b12-polyclonal-antibody-bs75574","provider":"AFSBio Inc.","version":"1.0","type":"link"}