{"product_id":"anti-human-cd3-fitc-cd19-pe-cocktail-e-ab-fc0004","title":"Anti-Human CD3-FITC\/CD19-PE Cocktail - E-AB-FC0004","description":"\u003cp\u003eAnti-Human CD3-FITC\/CD19-PE Cocktail\u003c\/p\u003e\n\n\u003cp\u003eSizes: 100 Tests, 100 Tests×2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-FC0004-100, E-AB-FC0004-100×2\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eConjugation: FITC, PE\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplication: FCM\u003c\/p\u003e\n\n\u003cp\u003eUsage: For whole blood samples, add 5 μL Anti-Human CD3-FITC\/CD19-PE Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.\u003cbr\u003e\nFor other samples, 1×10^6 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-FITC\/CD19-PE Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.\u003c\/p\u003e\n\n\u003cp\u003eClone No.: OKT-3, CB19\u003c\/p\u003e\n\n\u003cp\u003eBackground: This product is a FCM antibody cocktail made up of FITC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ) and PE Anti-Human CD19 Antibody [Clone: CB19] (Mouse IgG1, κ). \u003cbr\u003e\nCD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells. \u003cbr\u003e\nCD19 is a single-chain transmembrane glycoprotein expressed on B cells of all stages except plasma cells. It is a common marker for B cells. CD19 is also expressed in follicular dendritic cells. It forms complex with CD21 and CD84, which forms co-receptor with BCR. It takes part in B cell development, activantion and differentiation.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 5 μL\/Test\u003c\/p\u003e\n\n\u003cp\u003eBuffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This product can be stored at 2-8°C for 12 months. Please protect from prolonged exposure to light and do not freeze.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100 Tests","offer_id":43157163016371,"sku":"E-AB-FC0004-100","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100 Tests×2","offer_id":43157163049139,"sku":"E-AB-FC0004-100×2","price":427.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_3aaa211f-3114-4b73-8528-f7bab6ecade6.png?v=1710511811","url":"https:\/\/afsbio.com\/products\/anti-human-cd3-fitc-cd19-pe-cocktail-e-ab-fc0004","provider":"AFSBio Inc.","version":"1.0","type":"link"}