{"product_id":"ap2m1-polyclonal-antibody-bs7357","title":"AP2M1 Polyclonal Antibody-BS7357","description":"\u003cp\u003eAP2M1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS7357-50, BS7357-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96CW1\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eAP2M1 Polyclonal Antibody-BS7357\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:2000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The AP-2 coat assembly protein complex is an important component of clathrin-coated pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of α, β, μ, and σ protein subunits. The 50 kDa μ subunit (AP-2μ, AP2M1) is located at the core of the AP-2 complex and mediates interaction between the cargo protein and the clathrin-coated pit (1-4). The carboxy-terminal AP2M1 region recognizes the tyrosine-based, endocytotic sorting motif YXXφ found in cargo proteins and helps to bring the cargo protein to the clathrin-coated pit. Non-canonical, tyrosine-based endocytotic sorting signals can also promote interaction between cargo proteins and AP2M1 (5,6). AP2M1 plays an essential role in molecular signaling as it couples receptor-mediated endocytosis and pathways involving membrane receptors (7-9), matrix metalloproteinases (10), and ion channel proteins (11). Phosphorylation of specific AP2M1 residues and binding of lipids to this adaptor protein can regulate AP2M1 activity (12,13). Phosphorylation of AP2M1 at Thr156 by adaptor-associated kinase 1 (AAK1) stimulates affinity binding of AP2M1 to cargo protein signals (14).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: AP2M1 polyclonal antibody detects endogenous levels of AP2M1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~49 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of AP2M1 polyclonal antibody, Immunohistochemistry (IHC) analysis of AP2M1 polyclonal antibody in paraffin-embedded Rat Kidney tissue.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Adapter-related protein complex 2 mu subunit; \u003cbr\u003e\nAdaptin mu 1; \u003cbr\u003e\nAdaptin-mu2; \u003cbr\u003e\nAdaptor protein complex AP 2 subunit mu; \u003cbr\u003e\nAdaptor protein complex AP-2 subunit mu; \u003cbr\u003e\nAdaptor related protein complex 2 mu 1 subunit; \u003cbr\u003e\nAP 2 mu 2 chain; \u003cbr\u003e\nAP-2 complex subunit mu; \u003cbr\u003e\nAP-2 mu chain; \u003cbr\u003e\nAP2M 1; \u003cbr\u003e\nAp2m1; \u003cbr\u003e\nAP2M1_HUMAN; \u003cbr\u003e\nAP50; \u003cbr\u003e\nCLAPM1; \u003cbr\u003e\nClathrin adaptor complex AP2 mu subunit; \u003cbr\u003e\nClathrin assembly protein complex 2 medium chain; \u003cbr\u003e\nClathrin associated\/assembly\/adaptor protein medium 1; \u003cbr\u003e\nClathrin coat adaptor protein AP50; \u003cbr\u003e\nClathrin coat assembly protein AP50; \u003cbr\u003e\nClathrin coat associated protein AP50; \u003cbr\u003e\nClathrin coat-associated protein AP50; \u003cbr\u003e\nHA2 50 kDA subunit; \u003cbr\u003e\nmu2; \u003cbr\u003e\nPlasma membrane adaptor AP 2 50kDA protein; \u003cbr\u003e\nPlasma membrane adaptor AP-2 50 kDa protein;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant full length Human AP2M1.\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962818760883,"sku":"BS7357-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962818793651,"sku":"BS7357-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_096cc45d-1813-487e-83e6-f4509a564a84.jpg?v=1705319121","url":"https:\/\/afsbio.com\/products\/ap2m1-polyclonal-antibody-bs7357","provider":"AFSBio Inc.","version":"1.0","type":"link"}