{"product_id":"apaf-1-polyclonal-antibody-bs90065","title":"Apaf-1 Polyclonal Antibody-BS90065","description":"Apaf-1 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS90065-50, BS90065-100\n\nProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: O14727(Human) O88879(Mouse)\n\nHost: Rabbit\n\nReactivity: Human, Mouse\n\nApplications: WB, ICC\/IF, IHC\n\nAll Applications: WB:1:1,000\nICC:1:50-1:200\nIHC:1:50-1:200\n\nBackground: The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg\u003csup\u003e2+\u003c\/sup\u003e-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFkB in response to bacterial lipopoly-saccharides.\n\nPurification and Purity: ProA affinity purified\n\nStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\n\nSpecificity: Apaf-1 polyclonal antibody detects endogenous levels of Apaf-1 protein.\n\nBioworld Molecular Weight: 141 kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of Apaf-1 on HUVEC cell lysates using anti-Apaf-1 antibody at 1\/1,000 dilution., ICC staining Apaf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\n\nAlternative Name: APAF-1, APAF1, APAF 1, Apaf-1, APAF, Apaf1, Apoptotic peptidase activating factor 1, Apoptotic protease activating factor 1, Apoptotic protease activating factor, Apoptotic protease-activating factor 1, CED 4, CED4, KIAA0413, \n\nImmunogen: recombinant protein\n\nConjugate: Unconjugated\n\nModification: Unmodified","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979759620275,"sku":"BS90065-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979759653043,"sku":"BS90065-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_db15f9f1-8e0d-4bb8-ae4b-f866b7f26771.jpg?v=1705748751","url":"https:\/\/afsbio.com\/products\/apaf-1-polyclonal-antibody-bs90065","provider":"AFSBio Inc.","version":"1.0","type":"link"}