{"product_id":"apaf-1-polyclonal-antibody-bt-ap00501","title":"Apaf-1 Polyclonal Antibody - BT-AP00501","description":"\u003cp\u003eApaf-1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μL, 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BT-AP00501-20, BT-AP00501-50, BT-AP00501-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: APAF1 encodes a cytoplasmic protein that initiates apoptosis. Apoptotic protease-activating factor 1 contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cell Biology; Cancer\u003c\/p\u003e\n\n\u003cp\u003eTarget Protein: APAF1\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-p, ELISA\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eFull Product Name: Apaf-1 Antibody\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C for 1 year\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight (Da): 141840\u003c\/p\u003e\n\n\u003cp\u003eUniProt Accession: Human: O14727; Mouse: O88879; Rat: Q9EPV5\u003c\/p\u003e\n\n\u003cp\u003eSynonyms: APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1\u003c\/p\u003e\n\n\u003cp\u003eGeneID: Human: 317; Mouse: 11783; Rat: 78963\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"BT Lab","offers":[{"title":"20μL","offer_id":45240112185523,"sku":"BT-AP00501-20","price":151.2,"currency_code":"CAD","in_stock":true},{"title":"50μL","offer_id":45240112218291,"sku":"BT-AP00501-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45240112251059,"sku":"BT-AP00501-100","price":385.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_dd9ad4fc-dc34-4fce-9110-2fe5e9a7c800.png?v=1769403582","url":"https:\/\/afsbio.com\/products\/apaf-1-polyclonal-antibody-bt-ap00501","provider":"AFSBio Inc.","version":"1.0","type":"link"}