{"product_id":"apaf-1-rabbit-polyclonal-antibody-es4108","title":"Apaf-1 Rabbit Polyclonal Antibody - ES4108","description":"\u003cp\u003eApaf-1 Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES4108-50, ES4108-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: APAF1, KIAA0413, Apoptotic protease-activating factor 1, APAF-1\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: APAF1\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Apaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. IHC-p: 1\/100-1\/300. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Ubiquitous, Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung, Isoform 1 is expressed in heart, kidney and liver,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 135kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Platinum drug resistance, p53 signaling pathway, Apoptosis, Apoptosis - multiple species, Alzheimer disease, Parkinson disease, Amyotrophic lateral sclerosis, Huntington disease, Prion disease, Pathways of neurodegeneration - multiple diseases, Legionellosis, Tuberculosis, Hepatitis C, Hepatitis B, Measles, Influenza A, Herpes simplex virus 1 infection, Epstein-Barr virus infection, Pathways in cancer, Small cell lung cancer, Lipid and atherosclerosis\u003c\/p\u003e\n\n\u003cp\u003eFunction: Domain: The CARD domain mediates interaction with APIP\u003cbr\u003e\nFunction: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis\u003cbr\u003e\nInduction: By E2F and p53 in apoptotic neurons\u003cbr\u003e\nSimilarity: Contains 1 CARD domain\u003cbr\u003e\nSimilarity: Contains 1 NB-ARC domain\u003cbr\u003e\nSimilarity: Contains 13 WD repeats\u003cbr\u003e\nSubunit: Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP\u003cbr\u003e\nTissue Specificity: Ubiquitous. Highest levels of expression in adult spleen and per\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: APAF1\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Apoptotic protease-activating factor 1\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 11783\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: O88879\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 78963\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: Q9EPV5\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46316547604659,"sku":"ES4108-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46316547637427,"sku":"ES4108-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/apaf-1-rabbit-polyclonal-antibody-es4108","provider":"AFSBio Inc.","version":"1.0","type":"link"}