{"product_id":"arfaptin-1-k314-polyclonal-antibody-bs3076","title":"Arfaptin 1 (K314) Polyclonal Antibody-BS3076","description":"\u003cp\u003eArfaptin 1 (K314) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3076-50, BS3076-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P53367\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Arfaptin 1 (K314) polyclonal antibody detects endogenous levels of Arfaptin 1 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Arfaptin 1 (K314) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane 1: Hela cell lysate\u003cbr\u003e\nLane 2: SP2\/0 cell lysate\u003cbr\u003e\nLane 3: PC12 cell lysate\u003cbr\u003e\nLane 4: Rat muscle tissue lysate, Immunohistochemistry (IHC) analyzes of Arfaptin 1 (K314) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 40 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1; Arfaptin1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human Arfaptin 1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953232285875,"sku":"BS3076-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953232318643,"sku":"BS3076-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_281c3cb0-c861-433d-a969-6a8b37f3042b.jpg?v=1705057308","url":"https:\/\/afsbio.com\/products\/arfaptin-1-k314-polyclonal-antibody-bs3076","provider":"AFSBio Inc.","version":"1.0","type":"link"}