{"product_id":"ascl1-e158-polyclonal-antibody-bs3688","title":"ASCL1 (E158) Polyclonal Antibody-BS3688","description":"\u003cp\u003eASCL1 (E158) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3688-50, BS3688-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P50553\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Rat, Mouse, Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000~1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2\/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: ASCL1 (E158) polyclonal antibody detects endogenous levels of ASCL1 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of ASCL1 (E158) polyclonal antibody at 1:1000 dilution\u003cbr\u003e\nLane1:The brain tissue lysate of mouse(30ug)\u003cbr\u003e\nLane2:The brain tissue lysate of Rat(30ug)\u003cbr\u003e\nLane3:K562 whole cell lysate(30ug)\u003cbr\u003e\nLane4:BV2 whole cell lysate(30ug)\u003cbr\u003e\nLane5:A2780 cell membrane lysate(30ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 35 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Achaete-scute homolog 1; ASH-1; Hash1; Class A basic helix-loop-helix protein 46; bHLHa46\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human ASCL1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42908700410035,"sku":"BS3688-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42908700442803,"sku":"BS3688-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_36fa3447-f07a-4e9e-a058-05e68b5e7489.jpg?v=1703005980","url":"https:\/\/afsbio.com\/products\/ascl1-e158-polyclonal-antibody-bs3688","provider":"AFSBio Inc.","version":"1.0","type":"link"}