{"product_id":"ask-1-phospho-ser966-rabbit-pab-es1264","title":"ASK 1 (phospho Ser966) rabbit pAb - ES1264","description":"\u003cp\u003eASK 1 (phospho Ser966) rabbit pAb\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1264-50, ES1264-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase\/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase\/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)\/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK\/ERK. [provided by Re\u003c\/p\u003e\n\n\u003cp\u003eAlternate Name: MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK\/ERK kinase kinase 5; MEK kinase 5; MEKK 5\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; IHC; IF; ELISA\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human; Mouse; Rat\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966. AA range:932-981\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 155kD\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 4217\u003c\/p\u003e\n\n\u003cp\u003eHuman SWISS Prot NO: Q99683\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm. Endoplasmic reticulum. Interaction with 14-3-3 proteins alters the distribution of MAP3K5\/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"50μL","offer_id":45202004410547,"sku":"ES1264-50","price":207.2,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45202004443315,"sku":"ES1264-100","price":347.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_66ad812b-8144-436d-927d-1ced62829eef.png?v=1767119760","url":"https:\/\/afsbio.com\/products\/ask-1-phospho-ser966-rabbit-pab-es1264","provider":"AFSBio Inc.","version":"1.0","type":"link"}