{"product_id":"aspm-polyclonal-antibody-bs77601","title":"ASPM Polyclonal Antibody-BS77601","description":"ASPM Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS77601-50, BS77601-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200\n\nBackground: This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 350kDa\n\nImmunogen: A synthetic peptide of human ASPM(NP_060606.3).\n\nConjugate: Unconjugated\n\nModification: Unmodified \n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using ASPM antibody at 1:1000 dilution.\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\nLysates\/proteins: 25ug per lane.\nBlocking buffer: 3% nonfat dry milk in TBST.\nDetection: ECL Basic Kit.\nExposure time: 90s., Immunofluorescence analysis of C6 cells using ASPM antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using ASPM antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using ASPM antibody at dilution of 1:100. Blue: DAPI for nuclear staining.","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892340461747,"sku":"BS77601-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892340494515,"sku":"BS77601-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5f6a0eff-b327-4d0f-b1f7-c703dc8c92a5.jpg?v=1702483317","url":"https:\/\/afsbio.com\/products\/aspm-polyclonal-antibody-bs77601","provider":"AFSBio Inc.","version":"1.0","type":"link"}