{"product_id":"atg4a-polyclonal-antibody-bs62343","title":"ATG4A Polyclonal Antibody-BS62343","description":"\u003cp\u003eATG4A Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62343-50, BS62343-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8WYN0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg4a (ATG4 autophagy related 4 homolog A), also known as APG4A or AUTL2, is a 398 amino acid protein that localizes to the cytoplasm and belongs to the peptidase C54 family. Expressed in a variety of tissues, including brain, skeletal muscle and fetal liver, Atg4a functions as a cysteine protease that cleaves the C-terminal part of target proteins, such as GABARAP and MAP1LC3, and plays an essential role in autophagy. Atg4a exists as multiple alternatively spliced isoforms and is functionally inhibited by N-ethylmaleimide.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: ATG4A polyclonal antibody detects endogenous levels of ATG4A protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of ATG4A polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLaneA:HCT116 whole cell lysate\u003cbr\u003e\nLaneB:HepG2 whole cell lysate\u003cbr\u003e\nLaneC:CT26 whole cell lysate\u003cbr\u003e\nLaneD:PC12 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 60 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; ATG4A_HUMAN; Atg4al; AUT like 2 cysteine endopeptidase; AUT-like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase; Autophagy related cysteine endopeptidase 2; Autophagy related protein 4 homolog A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A;AV169859; Cysteine protease ATG4A; hAPG4A\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human ATG4A.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910445338803,"sku":"BS62343-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910445371571,"sku":"BS62343-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a814de2d-ebd2-48d4-94d7-5259bb236153.jpg?v=1703092523","url":"https:\/\/afsbio.com\/products\/atg4a-polyclonal-antibody-bs62343","provider":"AFSBio Inc.","version":"1.0","type":"link"}