{"product_id":"atg4a-polyclonal-antibody-bs7735","title":"ATG4A Polyclonal Antibody-BS7735","description":"\u003cp\u003eATG4A Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS7735-50, BS7735-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8WYN0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB\t1:1000 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg4a (ATG4 autophagy related 4 homolog A), also known as APG4A or AUTL2, is a 398 amino acid protein that localizes to the cytoplasm and belongs to the peptidase C54 family. Expressed in a variety of tissues, including brain, skeletal muscle and fetal liver, Atg4a functions as a cysteine protease that cleaves the C-terminal part of target proteins, such as GABARAP and MAP1LC3, and plays an essential role in autophagy. Atg4a exists as multiple alternatively spliced isoforms and is functionally inhibited by N-ethylmaleimide.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: ATG4A polyclonal antibody detects endogenous levels of ATG4A protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western Blot (WB) analysis of ATG4A pAb at 1:2000 dilution\u003cbr\u003e\n Lane1:CT26 whole cell lysate(20ug)\u003cbr\u003e\n Lane2:PC12 whole cell lysate(20ug)\u003cbr\u003e\n Lane3:HCT116 whole cell lysate(20ug)\u003cbr\u003e\n Lane4:PC3 whole cell lysate(20ug)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Apg4a; \u003cbr\u003e\nATG4 autophagy related 4 homolog A (S. cerevisiae); \u003cbr\u003e\nATG4A; \u003cbr\u003e\nATG4A_HUMAN; \u003cbr\u003e\nAtg4al; \u003cbr\u003e\nAUT like 2 cysteine endopeptidase; \u003cbr\u003e\nAUT-like 2 cysteine endopeptidase; \u003cbr\u003e\nAutl2; \u003cbr\u003e\nAutophagin 2; \u003cbr\u003e\nAutophagin-2; \u003cbr\u003e\nAutophagy related 4A cysteine peptidase; \u003cbr\u003e\nAutophagy related cysteine endopeptidase 2; \u003cbr\u003e\nAutophagy related protein 4 homolog A; \u003cbr\u003e\nAutophagy-related cysteine endopeptidase 2; \u003cbr\u003e\nAutophagy-related protein 4 homolog A; \u003cbr\u003e\nAV169859; \u003cbr\u003e\nCysteine protease ATG4A; \u003cbr\u003e\nhAPG4A;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombination protein , corresponding to amino acids 38-186 of Human ATG4A.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42923511742643,"sku":"BS7735-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42923511775411,"sku":"BS7735-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ae239b3a-6e93-44d0-bdaf-cedd655c67e0.jpg?v=1703850811","url":"https:\/\/afsbio.com\/products\/atg4a-polyclonal-antibody-bs7735","provider":"AFSBio Inc.","version":"1.0","type":"link"}