{"product_id":"atg5-polyclonal-antibody-bs90105","title":"ATG5 Polyclonal Antibody-BS90105","description":"\u003cp\u003eATG5 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90105-50, BS90105-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9H1Y0(Human) Q99J83(Mouse) Q3MQ06(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB:1:1,000-1:5,000\u003cbr\u003e\nICC:1:100-1:500\u003cbr\u003e\nIHC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: ATG5 polyclonal antibody detects endogenous levels of ATG5 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 55 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of ATG5 on different lysates using anti-ATG5 antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: Raji \u003cbr\u003e\n Lane 2: Hela \u003cbr\u003e\n Lane 3: PC12, ICC staining ATG5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ATG5, APG 5, APG 5L, APG5, APG5 autophagy 5 like, APG5 like, APG5-like, APG5L, Apoptosis specific protein, Apoptosis-specific protein, ASP, ATG 5, Atg5, ATG5 autophagy related 5 homolog, ATG5, Autophagy protein 5, Autophagy related 5, hAPG5, Homolog of S Cerevisiae autophagy 5, OTTHUMP00000040507,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979762110643,"sku":"BS90105-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979762143411,"sku":"BS90105-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_823b9edd-9936-4a75-b129-c46aca7f35c7.jpg?v=1705748834","url":"https:\/\/afsbio.com\/products\/atg5-polyclonal-antibody-bs90105","provider":"AFSBio Inc.","version":"1.0","type":"link"}