{"product_id":"atp5h-d146-polyclonal-antibody-bs3812","title":"ATP5H (D146) Polyclonal Antibody-BS3812","description":"\u003cp\u003eATP5H (D146) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3812-50, BS3812-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O75947\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIP: 1:10~1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: ATP5H (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit δ), also known as ATPQ, is a 161 amino acid protein that belongs to the ATPase δ subunit family. F-type ATPases, such as ATP5H, consist of two linked components: CF1, a soluble catalytic core that consists of five different subunits (α, β, γ, δ and ε), and CF0, a membrane proton channel that contains nine subunits (α, β, χ, δ, ε, φ, γ, F6 and 8). ATP5H encodes the δ subunit of the F0 complex. ATP5H produces ATP from ADP in the presence of a proton gradient across the membrane, which is generated by electron transport complexes of the respiratory chain. Localizing to mitochondrial inner membrane,\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: ATP5H (D146) polyclonal antibody detects endogenous levels of ATP5H protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 18 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of ATP5H (D146) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: Hela whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: MCF-7 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: H1792 whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: C6 whole cell lysate (40ug)\u003cbr\u003e\n Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ATP5H (D146) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of HEK293T cell lysate using ATP5H (D146) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using ATP5H (D146) #BS3812. “↑”（supernatant）;“↓(deposition）\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ATP synthase subunit d, mitochondrial; ATPase subunit d; ATP5H; ORF Names: My032\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 121-170 of Human ATP5H.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993786355891,"sku":"BS3812-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993786388659,"sku":"BS3812-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_bff2646c-bd33-44f6-ac51-ec7b8add07ca.jpg?v=1706183554","url":"https:\/\/afsbio.com\/products\/atp5h-d146-polyclonal-antibody-bs3812","provider":"AFSBio Inc.","version":"1.0","type":"link"}