{"product_id":"atp6v1h-polyclonal-antibody-bs62511","title":"ATP6V1H Polyclonal Antibody-BS62511","description":"\u003cp\u003eATP6V1H Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62511-50, BS62511-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9UI12\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000 IHC:1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis and an integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c’ and c”) make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The H subunit of V-ATPase, also designated SDF is comprised of two polypeptides derived from the same gene. This regulatory subunit plays a critical role in the functional coupling of ATP hydrolysis activity to proton transport in the V-ATPase pump.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: ATP6V1H polyclonal antibody detects endogenous levels of ATP6V1H protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of ATP6V1H polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLaneA:H9C2 whole cell lysate\u003cbr\u003e\nLaneB:MEF whole cell lysate\u003cbr\u003e\nLaneC:A549 whole cell lysate\u003cbr\u003e\nLaneD:HepG2 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 55 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: V-type proton ATPase subunit H; V-ATPase subunit H; Nef-binding protein 1; NBP1; Protein VMA13 homolog; V-ATPase 50\/57 kDa subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; ATP6V1H; CGI-11\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human ATP6V1H.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910448713907,"sku":"BS62511-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910448746675,"sku":"BS62511-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d9bee97a-94d9-4dfe-9f5c-577872da50d4.jpg?v=1703092605","url":"https:\/\/afsbio.com\/products\/atp6v1h-polyclonal-antibody-bs62511","provider":"AFSBio Inc.","version":"1.0","type":"link"}