{"product_id":"b2m-polyclonal-antibody-bs90115","title":"B2M Polyclonal Antibody-BS90115","description":"\u003cp\u003eB2M Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90115-50, BS90115-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P61769(Human) P01887(Mouse) P07151(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an a heavy chain that contains three subdomains (α1, α2, α3), and a non-covalent associating light chain, known as β-2-Microglobulin. β-2-Microglobulin associates with the a3 subdomain of the a heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The α1 and α2 domains of the a heavy chain form the peptide antigen-binding cleft. Mice that lack β-2-Microglobulin protein show a normal distribution of T cells, yet have no mature CD4-8+ T cells and are defective in CD4-8+ T cell-mediated cytotoxicity. Interferon-γ can stimulate production of β-2-Microglobulin transcripts. The human β-2-Microglobulin gene maps to chromosome 15q21-q22.2 and encodes a 119 amino acid protein. Mutations in the β-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: B2M polyclonal antibody detects endogenous levels of B2M protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 14 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of beta 2 Microglobulin on different lysates using anti-beta 2 Microglobulin antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: Raji \u003cbr\u003e\n Lane 2: U937, ICC staining beta 2 Microglobulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: B2M, B2M, B2MG, Beta 2 microglobin, Beta 2 microglobulin, Beta 2 microglobulin precursor, Beta chain of mhc class 1 proteins, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, CDABP0092, Hdcma22p,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979762733235,"sku":"BS90115-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979762766003,"sku":"BS90115-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d4a74285-c679-48de-846f-a66eb11b539a.jpg?v=1705748856","url":"https:\/\/afsbio.com\/products\/b2m-polyclonal-antibody-bs90115","provider":"AFSBio Inc.","version":"1.0","type":"link"}