{"product_id":"bak-polyclonal-antibody-bs79525","title":"Bak Polyclonal Antibody-BS79525","description":"\u003cp\u003eBak Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79525-50, BS79525-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q16611\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200   |   IP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 25KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of THP-1 cells, using Bak antibody at 1:1000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 180s., Immunofluorescence analysis of C6 cells using Bak Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH\/3T3 cells using Bak Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 300ug extracts of THP-1 cells using 3ug Bak antibody. Western blot was performed from the immunoprecipitate using Bak antibody at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: BAK1; BAK; BAK-LIKE; BCL2L7\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human Bak(NP_001179.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43050197844147,"sku":"BS79525-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43050197876915,"sku":"BS79525-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_cc93fe63-b866-419e-accb-87f6187425c2.jpg?v=1707912705","url":"https:\/\/afsbio.com\/products\/bak-polyclonal-antibody-bs79525","provider":"AFSBio Inc.","version":"1.0","type":"link"}