{"product_id":"bak-rabbit-monoclonal-antibody-bs9917m","title":"Bak Rabbit Monoclonal Antibody-BS9917M","description":"\u003cp\u003eBak Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9917M-50, BS9917M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q16611\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000 \u003cbr\u003e\nIHC\/ICC\/IF: 1:50-1:200\u003cbr\u003e\nFC: 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of Bak and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 23 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Bak Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: Hela whole cell lysate\u003cbr\u003e\n Lane 2: The skeletal muscle lysate of Human\u003cbr\u003e\n Lane 3: AGS whole cell lysate, Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin., ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Bcl-2 homologous antagonist\/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044033593523,"sku":"BS9917M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044033626291,"sku":"BS9917M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_04b19d62-a4d2-4fc9-a680-b1092595a436.jpg?v=1707738782","url":"https:\/\/afsbio.com\/products\/bak-rabbit-monoclonal-antibody-bs9917m","provider":"AFSBio Inc.","version":"1.0","type":"link"}