{"product_id":"bax-polyclonal-antibody-bs90120","title":"BAX Polyclonal Antibody-BS90120","description":"\u003cp\u003eBAX Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90120-50, BS90120-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q07812(Human) Q07813(Mouse) Q63690(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:1,000\u003cbr\u003e\nICC:1:200\u003cbr\u003e\nIHC:1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Peptide affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: BAX polyclonal antibody detects endogenous levels of BAX protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 21 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1\/500 dilution. \u003cbr\u003e\n Positive control:\u003cbr\u003e\n Lane 1: HepG2 \u003cbr\u003e\n Lane 2: Hela \u003cbr\u003e\n Lane 3: Mouse embryonic stem cell \u003cbr\u003e\n Lane 4: PC12 \u003cbr\u003e\n Lane 5: F9, ICC staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Bcl2-L-4, Apoptosis regulator BAX, BAX, Bax-protein, BAX, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979763060915,"sku":"BS90120-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979763093683,"sku":"BS90120-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_595a4704-6d5d-4a74-87bc-fdcbbc39651a.jpg?v=1705748868","url":"https:\/\/afsbio.com\/products\/bax-polyclonal-antibody-bs90120","provider":"AFSBio Inc.","version":"1.0","type":"link"}