{"product_id":"bcl-2-p65-polyclonal-antibody-ap0368","title":"Bcl-2 (P65) Polyclonal Antibody-AP0368","description":"\u003cp\u003eBcl-2 (P65) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0368-50, AP0368-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q16611\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:1000~1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Bak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Bcl-2 (P65) polyclonal antibody detects endogenous levels of Bcl-2 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 30 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Bcl-2 (P65) polyclonal antibody at 1:1000 dilution\u003cbr\u003e\n Lane 1: The Kidney tissue lysate of Rat (30ug)\u003cbr\u003e\n Lane 2: The Heart tissue lysate of Mouse (30ug), Western blot (WB) analysis of Bcl-2 (P65) pAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: The Kidney tissue lysate of Rat (30ug)\u003cbr\u003e\n Lane 2: A2780 whole cell lysate (30ug)\u003cbr\u003e\n Lane 3: A549 whole cell lysate (30ug)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Bcl-2 homologous antagonist\/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human Bcl-2.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989589725363,"sku":"AP0368-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989589758131,"sku":"AP0368-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ce84b685-c698-45e7-af90-7ceb20a24263.jpg?v=1706095853","url":"https:\/\/afsbio.com\/products\/bcl-2-p65-polyclonal-antibody-ap0368","provider":"AFSBio Inc.","version":"1.0","type":"link"}