{"product_id":"bcr-d171-polyclonal-antibody-bs1033","title":"Bcr (D171) Polyclonal Antibody-BS1033","description":"\u003cp\u003eBcr (D171) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1033-50, BS1033-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P11274\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB IHC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Bcr gene, mapping on chromosome 22, was initially identified on the basis of its fusion with the c-Abl proto-oncogene on chromosome 9 resulting in the generation of the Philadelphia chromosome in 90-95% of patients with chronic myelogenous leukemia (CML). The Bcr gene encodes for the Breakpoint cluster region protein (Bcr). A consequence of this translocation is the generation of a Bcr\/c-Abl mRNA encoding an activated c-Abl protein kinase. The Bcr gene has been shown to encode a 160 kDa GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, Rac 1 p21. While it has been speculated that the Bcr protein may also stimulate Rac 2 p21 GTPase activity, it has no effect on Ras p21 or Rho p21 GTPases.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Bcr (D171) polyclonal antibody detects endogenous levels of Bcr protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Bcr (D171) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane1:AML-12 whole cell lysate(40ug)\u003cbr\u003e\nLane2:PC12 whole cell lysate(40ug)\u003cbr\u003e\nLane3:MCF-7 whole cell lysate(40ug)\u003cbr\u003e\nLane4:HepG2 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 160 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Breakpoint cluster region protein; Renal carcinoma antigen NY-REN-26; BCR; BCR1; D22S11\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 141-190 of Human Bcr.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910474371251,"sku":"BS1033-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910474404019,"sku":"BS1033-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_33a34308-5eeb-421b-a6b3-74e89e8bba97.jpg?v=1703093134","url":"https:\/\/afsbio.com\/products\/bcr-d171-polyclonal-antibody-bs1033","provider":"AFSBio Inc.","version":"1.0","type":"link"}