{"product_id":"beclin-1-monoclonal-antibody-mb67099","title":"Beclin-1 Monoclonal Antibody - MB67099","description":"\u003cp\u003eBeclin-1 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB67099-50, MB67099-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions. One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6\/Vps30. Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimμlate autophagy when overexpressed in mammalian cells. Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak. While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers . Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export . Investigators have demonstrated that Beclin-1-\/- mice die early in embryogenesis and Beclin-1-\/+ mice have a high incidence of spontaneous tumors. Stem cells from the nμll mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal. Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resμlted in significant protection against Sindbis virus-induced disease and neuronal apoptosis.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human Beclin-1. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/500 - 1\/1000), IHC (1\/50 - 1\/200), IF\/ICC (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of Beclin-1 protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 57 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Autophagy Signaling\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: Q14457\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG3 kappa. Supplied in crude ascites with 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946158776499,"sku":"MB67099-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946158809267,"sku":"MB67099-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/beclin-1-monoclonal-antibody-mb67099","provider":"AFSBio Inc.","version":"1.0","type":"link"}